Dengue fever is a tropical viral infectious disease caused by the dengue virus, primarily transmitted to humans through mosquitoes. It is widely prevalent globally, causing millions of infections and thousands of deaths every year. Symptoms of dengue fever include high fever, headaches, joint and muscle pain, and in severe cases, it can lead to bleeding and organ damage. Due to its rapid and widespread transmission, dengue fever poses a significant threat to public health and global well-being.
To promptly diagnose and control the spread of dengue fever, rapid and accurate virus testing has become crucial. In this regard, rapid diagnostic kits play a vital role. They are user-friendly, swift testing tools that assist medical institutions and epidemiological investigators in quickly determining whether individuals are carrying the dengue virus. By employing these diagnostic kits, doctors and researchers can diagnose and isolate infected individuals earlier, implement appropriate treatment and control measures, thus effectively curbing the spread of dengue fever. Therefore, rapid diagnostic kits hold significant importance in the prevention and control of dengue fever outbreaks.
Working Principle and Usage Procedure of Rapid Diagnostic Kit
· Basic Principles of Antibody-Antigen Reaction
The antibody-antigen reaction is a fundamental principle in immunology used for specific recognition and binding of antigens. Antibodies bind to antigens to form immune complexes, a binding process driven by mutual attraction and affinity between antibodies and antigens. In the context of the dengue fever test kit, antibodies bind to antigens from the dengue virus, resulting in the formation of visible immune complexes.
· Assay Procedure of the Diagnostic Kit
Step 1: Bring the specimen and test components to room temperature if refrigerated or frozen. Once thawed, mix the specimen well prior to assay.
Step 2: When ready to test, open the pouch at the notch and remove device. Place the test device on a clean, flat surface.
Step 3: Be sure to label the device with specimen’s ID number.
Step 4: For whole blood test
- Apply 1 drop of whole blood (about 30-35 µL) into the sample well.
- Then add 2 dropS (about 60-70 µL) of Sample Diluent immediately.
For serum or plasma test
- Fill the pipette dropper with the specimen.
- Holding the dropper vertically, dispense 1 drop (about 30-35 µL) of specimen into the sample well making sure that there are no air bubbles.
-Then add 2 dropS (about 60-70 µL) of Sample Diluent immediately.
Step 6: Results can be read in 20 minutes. Positive results can be visible in as short as 1 minute.
Don’t read results after 30 minutes.To avoid confusion, discard the test device after interpreting the result.
· Interpretation of Assay Result
1. NEGATIVE RESULT: If only the C band is developed, the test indicates that the level of dengue Ag in the specimen is undetectable. The result is negative or non-reactive.
2. POSITIVE RESULT: If both C and T bands are developed, the test indicates that the specimen contains dengue Ag. The result is positive or reactive.Samples with positive results should be confirmed with alternative testing method(s) such as PCR or ELISA and clinical findings before a positive determination is made.
3. INVALID: If no C band is developed, the assay is invalid regardless of color development on the T band as indicated below. Repeat the assay with a new device.
Advantages of BoatBio Dengue Rapid Diagnostic Kit
· Rapidity
1. Reduced Testing Time:
The diagnostic kit employs rapid testing technology, allowing sample analysis and result generation to be completed within 20 minutes.
Compared to traditional laboratory methods, the kit significantly shortens the testing time, enhancing work efficiency.
2. Real-Time Result Obtaining:
The diagnostic kit provides real-time results immediately after sample processing and reaction completion.
This enables medical professionals to quickly make diagnoses and decisions, expediting disease assessment and treatment processes.
· Sensitivity and Specificity
1. Strong Sensitivity:
The kit’s design enables it to detect the presence of dengue virus with high sensitivity.
Even in samples with low virus concentrations, the kit reliably detects the virus, enhancing diagnostic accuracy.
2. High Specificity:
The kit’s antibodies exhibit high specificity, allowing them to bind specifically to the dengue virus.
This differentiation capability enables the kit to distinguish between dengue virus infection and other related viruses
(such as Zika virus, yellow fever virus), minimizing misdiagnosis and false negatives.
· Ease of Use
1. Simple Operational Steps:
The diagnostic kit typically features straightforward operational steps, enabling users to quickly familiarize themselves with its use.
Clear and concise steps are involved, including sample addition, reagent mixing, reaction, and result interpretation.
2. No Need for Complex Equipment or Lab Conditions:
The diagnostic kit generally doesn’t require complex equipment or lab conditions for operation and result reading.
This portability and flexibility make the kit suitable for various scenarios, including remote areas or healthcare facilities with limited resources.
In summary, the Dengue Rapid Diagnostic Kit offers advantages such as rapidity, sensitivity, specificity, and ease of use, making it a valuable tool for efficient and accurate dengue virus detection in diverse settings.
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Post time: Aug-16-2023